2 publications
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Dual Modification of a Triple-Stranded β-Helix Nanotube with Ru and Re Metal Complexes to Promote Photocatalytic Reduction of CO2
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Chem. Commun. 2011, 47, 2074, 10.1039/C0CC03015E
We have constructed a robust β-helical nanotube from the component proteins of bacteriophage T4 and modified this nanotube with RuII(bpy)3 and ReI(bpy)(CO)3Cl complexes. The photocatalytic system arranged on the tube catalyzes the reduction of CO2 with higher reactivity than that of the mixture of the monomeric forms.
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Metal: RuLigand type: BipyridineHost protein: [(gp5βf)3]2Anchoring strategy: Lysine-succinimideOptimization: GeneticNotes: ---
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Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme
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ACS Catal. 2021, 11, 5628-5635, 10.1021/acscatal.1c00287
Photosystem I (PSI) is a very large membrane protein complex (∼1000 kDa) harboring P700*, the strongest reductant known in biological systems, which is responsible for driving NAD(P)+ and ultimately for CO2 reduction. Although PSI is one of the most important components in the photosynthesis machinery, it has remained difficult to enhance PSI functions through genetic engineering due to its enormous complexity. Inspired by PSI’s ability to undergo multiple-step photo-induced electron hopping from P700* to iron–sulfur [Fe4S4] clusters, we designed a 33 kDa miniature photocatalytic CO2-reducing enzyme (mPCE) harboring a chromophore (BpC) and two [Fe4S4] clusters (FeA/FeB). Through reduction potential fine-tuning, we optimized the multiple-step electron hopping from BpC to FeA/FeB, culminating in a CO2/HCOOH conversion quantum efficiency of 1.43%. As mPCE can be overexpressed with a high yield in Escherichia coli cells without requiring synthetic cofactors, further development along this route may result in rapid photo-enzyme quantum yield improvement and functional expansion through an efficient directed evolution process.
Metal: FeLigand type: Amino acidHost protein: Ferredoxin (Fd)Anchoring strategy: DativeOptimization: GeneticNotes: ---