Synthesis of a Sequence-Specific DNA-Cleaving Peptide
Science 1987, 238, 1129-1132, 10.1126/science.3120311
A synthetic 52-residue peptide based on the sequence-specific DNA-binding domain of Hin recombinase (139-190) has been equipped with ethylenediaminetetraacetic acid (EDTA) at the amino terminus. In the presence of Fe(II), this synthetic EDTA-peptide cleaves DNA at Hin recombination sites. The cleavage data reveal that the amino terminus of Hin(139-190) is bound in the minor groove of DNA near the symmetry axis of Hin recombination sites. This work demonstrates the construction of a hybrid peptide combining two functional domains: sequence-specific DNA binding and DNA cleavage.
Metal: FeLigand type: EDTAHost protein: Domain of Hin recombinaseAnchoring strategy: CovalentOptimization: ---Reaction: DNA cleavageMax TON: <1ee: ---PDB: ---Notes: Engineered sequence specificity