2 publications
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A "Broad Spectrum" Carbene Transferase for Synthesis of Chiral α-Trifluoromethylated Organoborons
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ACS Cent. Sci. 2019, 5, 206-208, 10.1021/acscentsci.9b00015
Directed evolution generated an enzyme for the enantioselective synthesis of α-trifluoromethylated organoborons—potentially attractive synthons for fluorinated compounds.
Metal: FeLigand type: PorphyrinHost protein: Cytochrome cAnchoring strategy: NativeOptimization: GeneticNotes: ---
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Nitrene Transfer Catalyzed by a Non-Heme Iron Enzyme and Enhanced by Non-Native Small-Molecule Ligands
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J. Am. Chem. Soc. 2019, 141, 19585-19588, 10.1021/jacs.9b11608
Transition-metal catalysis is a powerful tool for the construction of chemical bonds. Here we show that Pseudomonas savastanoi ethylene-forming enzyme, a non-heme iron enzyme, can catalyze olefin aziridination and nitrene C−H insertion, and that these activities can be improved by directed evolution. The nonheme iron center allows for facile modification of the primary coordination sphere by addition of metalcoordinating molecules, enabling control over enzyme activity and selectivity using small molecules.
Metal: FeLigand type: Amino acidHost protein: Pseudomonas savastanoi ethylene-forming enzyme (PsEFE)Anchoring strategy: NativeOptimization: GeneticNotes: Additional reaction: aziridination