22 publications

22 publications

A Hydrogenase Model System Based on the Sequence of Cytochrome c: Photochemical Hydrogen Evolution in Aqueous Media

Hayashi, T

Chem. Commun., 2011, 10.1039/c1cc11157d

The diiron carbonyl cluster is held by a native CXXC motif, which includes Cys14 and Cys17, in the cytochrome c sequence. It is found that the diiron carbonyl complex works well as a catalyst for H2 evolution. It has a TON of ∼80 over 2 h at pH 4.7 in the presence of a Ru-photosensitizer and ascorbate as a sacrificial reagent in aqueous media.


Metal: Fe
Ligand type: Carbonyl
Host protein: Cytochrome c
Anchoring strategy: Dative
Optimization: ---
Reaction: H2 evolution
Max TON: 82
ee: ---
PDB: ---
Notes: Horse heart cytochrome C

An Artificial Metalloenzyme for Olefin Metathesis

Hilvert, D.; Ward, T. R.

Chem. Commun., 2011, 10.1039/c1cc15005g

A Grubbs–Hoveyda type olefin metathesis catalyst, equipped with an electrophilic bromoacetamide group, was used to modify a cysteine-containing variant of a small heat shock protein from Methanocaldococcus jannaschii. The resulting artificial metalloenzyme was found to be active under acidic conditions in a benchmark ring closing metathesis reaction.


Metal: Ru
Ligand type: Carbene
Anchoring strategy: Covalent
Optimization: ---
Reaction: Olefin metathesis
Max TON: 25
ee: ---
PDB: ---
Notes: RCM

Aqueous Light Driven Hydrogen Production by a Ru–Ferredoxin–Co Biohybrid

Utschig, L. M.

Chem. Commun., 2015, 10.1039/c5cc03006d


Metal: Co
Ligand type: Oxime
Host protein: Ferredoxin (Fd)
Anchoring strategy: Dative
Optimization: ---
Reaction: H2 evolution
Max TON: 210
ee: ---
PDB: ---
Notes: Recalculated TON

A Rhodium Complex-Linked β-Barrel Protein as a Hybrid Biocatalyst for Phenylacetylene Polymerization

Hayashi, T

Chem. Commun., 2012, 10.1039/C2CC35165J

Our group recently prepared a hybrid catalyst containing a rhodium complex, Rh(Cp)(cod), with a maleimide moiety at the peripheral position of the Cp ligand. This compound was then inserted into a β-barrel protein scaffold of a mutant of aponitrobindin (Q96C) via a covalent linkage. The hybrid protein is found to act as a polymerization catalyst and preferentially yields trans-poly(phenylacetylene) (PPA), although the rhodium complex without the protein scaffold normally produces cis PPA.


Metal: Rh
Ligand type: COD; Cp*
Host protein: Nitrobindin (Nb)
Anchoring strategy: Cystein-maleimide
Optimization: ---
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Artificial Metalloenzymes for Olefin Metathesis Based on the Biotin-(Strept)Avidin Technology

Ward, T. R.

Chem. Commun., 2011, 10.1039/c1cc15004a


Metal: Ru
Ligand type: Carbene
Host protein: Streptavidin (Sav)
Anchoring strategy: Supramolecular
Optimization: Chemical
Reaction: Olefin metathesis
Max TON: 14
ee: ---
PDB: ---
Notes: RCM

Metal: Ru
Ligand type: Carbene
Host protein: Avidin (Av)
Anchoring strategy: Supramolecular
Optimization: Chemical
Reaction: Olefin metathesis
Max TON: 19
ee: ---
PDB: ---
Notes: RCM

Chemical Optimization of Artificial Metalloenzymes Based on the Biotin-Avidin Technology: (S)-Selective and Solvent-Tolerant Hydrogenation Catalysts via the Introduction of Chiral Amino Acid Spacers

Ward, T. R.

Chem. Commun., 2005, 10.1039/b509015f


Metal: Rh
Ligand type: Phosphine
Host protein: Streptavidin (Sav)
Anchoring strategy: Supramolecular
Optimization: Chemical
Reaction: Hydrogenation
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Chemogenetic Protein Engineering: An Efficient Tool for the Optimization of Artificial Metalloenzymes

Review

Ward, T. R.

Chem. Commun., 2008, 10.1039/b806652c


Notes: ---

Creation of an Artificial Metalloprotein with a Hoveyda–Grubbs Catalyst Moiety through the Intrinsic Inhibition Mechanism of α-Chymotrypsin

Chem. Commun., 2012, 10.1039/c2cc16898g


Metal: Ru
Ligand type: Carbene
Host protein: α-chymotrypsin
Anchoring strategy: Covalent
Optimization: ---
Reaction: Olefin metathesis
Max TON: 20
ee: ---
PDB: ---
Notes: RCM

Definite Coordination Arrangement of Organometallic Palladium Complexes Accumulated on the Designed Interior Surface of Apo-Ferritin

Ueno, T.

Chem. Commun., 2011, 10.1039/C0CC02221G


Metal: Pd
Ligand type: Allyl
Host protein: Ferritin
Anchoring strategy: Dative
Optimization: Genetic
Reaction: Suzuki coupling
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Directed Evolution of Hybrid Enzymes: Evolving Enantioselectivity of an Achiral Rh-Complex Anchored to a Protein

Reetz, M. T.

Chem. Commun., 2006, 10.1039/b610461d


Metal: Rh
Ligand type: COD; Phosphine
Host protein: Streptavidin (Sav)
Anchoring strategy: Supramolecular
Optimization: Genetic
Reaction: Hydrogenation
Max TON: 4500
ee: 65
PDB: ---
Notes: ---

Dual Modification of a Triple-Stranded β-Helix Nanotube with Ru and Re Metal Complexes to Promote Photocatalytic Reduction of CO2

Ueno, T.

Chem. Commun., 2011, 10.1039/C0CC03015E


Metal: Re
Ligand type: Bipyridine; CO
Host protein: [(gp5βf)3]2
Anchoring strategy: Cystein-maleimide
Optimization: ---
Reaction: CO2 reduction
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Metal: Ru
Ligand type: Bipyridine
Host protein: [(gp5βf)3]2
Anchoring strategy: Lysine-succinimide
Optimization: Genetic
Reaction: CO2 reduction
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Enantioselective Sulfoxidation Mediated by Vanadium-Incorporated Phytase: A Hydrolase Acting as a Peroxidase

Sheldon, R. A.

Chem. Commun., 1998, 10.1039/a804702b


Metal: V
Ligand type: Undefined
Host protein: Phytase
Anchoring strategy: Undefined
Optimization: ---
Reaction: Sulfoxidation
Max TON: ~194
ee: 66
PDB: ---
Notes: ---

Metal: V
Ligand type: Oxide
Host protein: Phytase
Anchoring strategy: Undefined
Optimization: ---
Reaction: Sulfoxidation
Max TON: 550
ee: 66
PDB: ---
Notes: ---

Enantioselective Transfer Hydrogenation of Ketone Catalysed by Artificial Metalloenzymes Derived from Bovine β-Lactoglobulin

Salmain, M.

Chem. Commun., 2012, 10.1039/c2cc36980j


Metal: Rh
Ligand type: Cp*; Poly-pyridine
Host protein: ß-lactoglobulin
Anchoring strategy: Supramolecular
Optimization: Chemical
Reaction: Hydrogenation
Max TON: 34
ee: 26
PDB: ---
Notes: ---

From "Hemoabzymes" to "Hemozymes": Towards new Biocatalysts for Selective Oxidations

Review

Mahy, J.-P.

Chem. Commun., 2015, 10.1039/c4cc08169b


Notes: ---

Immobilization of an Artificial Imine Reductase Within Silica Nanoparticles Improves its Performance

Shahgaldian, P.; Ward, T. R.

Chem. Commun., 2016, 10.1039/c6cc04604e


Metal: Ir
Ligand type: Amino-sulfonamide; Cp*
Host protein: Streptavidin (Sav)
Anchoring strategy: Supramolecular
Optimization: Genetic
Max TON: 4554
ee: 89
PDB: ---
Notes: Reaction in nanoparticles

Immobilization of Two Organometallic Complexes into a Single Cage to Construct Protein-Based Microcompartment

Ueno, T.

Chem. Commun., 2016, 10.1039/C6CC00679E


Metal: Ir
Ligand type: Amino acid; Cp*
Host protein: Apo-ferritin
Anchoring strategy: Dative
Optimization: Chemical
Reaction: Hydrogenation
Max TON: ~2
ee: 15
PDB: 5E2D
Notes: Tandem reaction (Hydrogenation and Suzuki-Miyaura coupling) to form biphenylethanol from 4-iodoacetophenone and phenylboronic acid. TON and ee are given for the tandem reaction product.

Metal: Pd
Ligand type: Allyl; Amino acid
Host protein: Apo-ferritin
Anchoring strategy: Dative
Optimization: Chemical
Max TON: ~1
ee: 15
PDB: 5E2D
Notes: Tandem reaction (Hydrogenation and Suzuki-Miyaura coupling) to form biphenylethanol from 4-iodoacetophenone and phenylboronic acid.

Lipase Active Site Covalent Anchoring of Rh(NHC) Catalysts: Towards Chemoselective Artificial Metalloenzymes

Klein Gebbink, R. J. M.

Chem. Commun., 2015, 10.1039/c4cc09700a


Metal: Rh
Ligand type: Carbene
Host protein: Cutinase
Anchoring strategy: Covalent
Optimization: ---
Reaction: Hydrogenation
Max TON: 20
ee: rac.
PDB: 1CEX
Notes: ---

Metal: Rh
Ligand type: Carbene
Anchoring strategy: Covalent
Optimization: ---
Reaction: Hydrogenation
Max TON: 20
ee: rac.
PDB: 4K6G
Notes: ---

Merging Homogeneous Catalysis with Biocatalysis; Papain as Hydrogenation Catalyst

de Vries, J.

Chem. Commun., 2005, 10.1039/B512138H


Metal: Rh
Ligand type: Phosphine
Host protein: Papain (PAP)
Anchoring strategy: Covalent
Optimization: ---
Reaction: Hydrogenation
Max TON: 400
ee: <10
PDB: ---
Notes: ---

Merging the Best of Two Worlds: Artificial Metalloenzymes for Enantioselective Catalysis

Review

Ward, T. R.

Chem. Commun., 2011, 10.1039/c1cc11592h


Notes: ---

Protein Scaffold of a Designed Metalloenzyme Enhances the Chemoselectivity in Sulfoxidation of Thioanisole

Lu, Y.

Chem. Commun., 2008, 10.1039/b718915j


Metal: Mn
Ligand type: Salen
Host protein: Myoglobin (Mb)
Anchoring strategy: Supramolecular
Optimization: Chemical & genetic
Reaction: Sulfoxidation
Max TON: 5.2
ee: 60
PDB: ---
Notes: Sperm whale myoglobin

Protein Secondary-Shell Interactions Enhance the Photoinduced Hydrogen Production of Cobalt Protoporphyrin IX

Ghirlanda, G.

Chem. Commun., 2014, 10.1039/c4cc06700b


Metal: Co
Ligand type: Porphyrin
Host protein: Myoglobin (Mb)
Anchoring strategy: Metal substitution
Optimization: Genetic
Reaction: H2 evolution
Max TON: 518
ee: ---
PDB: ---
Notes: ---

Synthesis of a Heterogeneous Artificial Metallolipase with Chimeric Catalytic Activity

Filice, M.

Chem. Commun., 2015, 10.1039/C5CC02450A


Metal: Cu
Ligand type: Phenanthroline
Anchoring strategy: Covalent
Optimization: Genetic
Max TON: 411
ee: 92
PDB: ---
Notes: ArM is immobilized on Sepabeads. Endo/exo = 93.5%

Metal: Cu
Ligand type: Phenanthroline
Anchoring strategy: Covalent
Optimization: Genetic
Reaction: Reduction
Max TON: ---
ee: ---
PDB: ---
Notes: Cascade reaction: Ester hydrolysis (natural function of the host protein) followed by reduction (function of the designed ArM).