5 publications

5 publications

A Structural View of Synthetic Cofactor Integration into [FeFe]-Hydrogenases

Apfel, U.-P.; Happe, T.; Kurisu, G.

Chem. Sci., 2016, 10.1039/C5SC03397G


Metal: Fe
Ligand type: CN; CO; Dithiolate
Anchoring strategy: Dative
Optimization: Chemical
Reaction: H2 evolution
Max TON: ---
ee: ---
PDB: 4XDC
Notes: H2 evolution activity of the ArM: 2874 (mmol H2)*min-1*(mg protein)-1.

Chalcogenide Substitution in the [2Fe] Cluster of [FeFe]-Hydrogenases Conserves High Enzymatic Activity

Apfel, U.-P.; Happe, T.

Dalton Trans., 2017, 10.1039/C7DT03785F


Metal: Fe
Ligand type: CN; CO; Diselenolate
Anchoring strategy: Dative
Optimization: Chemical
Reaction: H2 evolution
Max TON: ---
ee: ---
PDB: 5OEF
Notes: ---

Generation of a Functional, Semisynthetic [FeFe]-Hydrogenase in a Photosynthetic Microorganism

Berggren, G.; Lindblad, P.

Energy Environ. Sci., 2018, 10.1039/C8EE01975D


Metal: Fe
Ligand type: CN; CO
Anchoring strategy: Reconstitution
Optimization: Chemical & genetic
Reaction: H2 evolution
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Hybrid [FeFe]-Hydrogenases with Modified Active Sites Show Remarkable Residual Enzymatic Activity

Lubitz, W.; Reijerse, E.

Biochemistry, 2015, 10.1021/bi501391d


Metal: Fe
Ligand type: CN; CO; Dithiolate
Anchoring strategy: Dative
Optimization: Chemical
Max TON: ---
ee: ---
PDB: ---
Notes: H2 evolution: TOF = 450 s-1. H2 oxidation: TOF = 150 s-1.

Spontaneous Activation of [FeFe]-Hydrogenases by an Inorganic [2Fe] Active Site Mimic

Happe, T.

Nat. Chem. Biol., 2013, 10.1038/Nchembio.1311


Metal: Fe
Ligand type: CN; CO; Dithiolate
Anchoring strategy: Dative
Optimization: Chemical
Reaction: H2 evolution
Max TON: ---
ee: ---
PDB: ---
Notes: ---