1 publication

1 publication

Generation of a Hybrid Sequence-Specific Single Stranded Deoxyribonuclease

Schultz, P.G.

Science 1987, 238, 1401-1403, 10.1126/science.3685986

The relatively nonspecific single-stranded deoxyribonuclease, staphylococcal nuclease, was selectively fused to an oligonucleotide binding site of defined sequence to generate a hybrid enzyme. A cysteine was substituted for Lys116 in the enzyme by oligonucleotide-directed mutagenesis and coupled to an oligonucleotide that contained a 3'-thiol. The resulting hybrid enzyme cleaved single-stranded DNA at sites adjacent to the oligonucleotide binding site.

Metal: Ca
Ligand type: Undefined
Host protein: Staphylococcal nuclease
Anchoring strategy: ---
Optimization: ---
Max TON: <1
ee: ---
PDB: ---
Notes: Engineered sequence specificity