1 publication

1 publication

Metal-Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes

Eppinger, J.

ChemistryOpen 2013, 2, 50-54, 10.1002/open.201200044

How to train a protein: Metal‐conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82 % e.r. in the hydrogenation of ketones. The modular setup enables a rapid generation of artificial metalloenzyme libraries, which can be adapted to a broad range of catalytic conditions.


Metal: Rh
Ligand type: Cp*; Phosphine
Host protein: Papain (PAP)
Anchoring strategy: Covalent
Optimization: Chemical
Reaction: Hydrogenation
Max TON: 89
ee: 64
PDB: ---
Notes: ---

Metal: Ru
Ligand type: Benzene; Phosphine
Host protein: Bromelain
Anchoring strategy: Covalent
Optimization: Chemical
Reaction: Hydrogenation
Max TON: 44
ee: 20
PDB: ---
Notes: ---