11 publications

11 publications

A Metal Ion Regulated Artificial Metalloenzyme

Roelfes, G.

Dalton Trans., 2017, 10.1039/C7DT00533D

Regulation of enzyme activity is essential in living cells. The rapidly increasing number of designer enzymes with new-to-nature activities makes it necessary to develop novel strategies for controlling their catalytic activity. Here we present the development of a metal ion regulated artificial metalloenzyme created by combining two anchoring strategies, covalent and supramolecular, for introducing a regulatory and a catalytic site, respectively. This artificial metalloenzyme is activated in the presence of Fe2+ ions, but only marginally in the presence of Zn2+.


Metal: Fe
Ligand type: Bypyridine
Host protein: LmrR
Anchoring strategy: Covalent
Optimization: Genetic
Max TON: 14
ee: 75
PDB: ---
Notes: ---

Metal: Zn
Ligand type: Bypyridine
Host protein: LmrR
Anchoring strategy: Covalent
Optimization: Genetic
Max TON: 6
ee: 80
PDB: ---
Notes: ---

Artificial Metalloenzymes Derived from Bovine β-Lactoglobulin for the Asymmetric Transfer Hydrogenation of an Aryl Ketone – Synthesis, Characterization and Catalytic Activity

Salmain, M.

Dalton Trans., 2014, 10.1039/c3dt53253d


Metal: Rh
Ligand type: Cp*; Poly-pyridine
Host protein: ß-lactoglobulin
Anchoring strategy: Supramolecular
Optimization: Chemical
Reaction: Hydrogenation
Max TON: 14
ee: 32
PDB: ---
Notes: ---

Bovine Serum Albumin-Cobalt(II) Schiff Base Complex Hybrid: An Efficient Artificial Metalloenzyme for Enantioselective Sulfoxidation using Hydrogen Peroxide

Bian, H.-D.; Liang, H.

Dalton Trans., 2016, 10.1039/C5DT04507J


Metal: Co
Ligand type: Amine; Phenolate
Anchoring strategy: Supramolecular
Optimization: Chemical
Reaction: Sulfoxidation
Max TON: 98
ee: 87
PDB: ---
Notes: ---

Chalcogenide Substitution in the [2Fe] Cluster of [FeFe]-Hydrogenases Conserves High Enzymatic Activity

Apfel, U.-P.; Happe, T.

Dalton Trans., 2017, 10.1039/C7DT03785F


Metal: Fe
Ligand type: CN; CO; Diselenolate
Anchoring strategy: Dative
Optimization: Chemical
Reaction: H2 evolution
Max TON: ---
ee: ---
PDB: 5OEF
Notes: ---

Ferritin Encapsulation of Artificial Metalloenzymes: Engineering a Tertiary Coordination Sphere for an Artificial Transfer Hydrogenase

Ward, T. R.

Dalton Trans., 2018, 10.1039/C8DT02224K


Metal: Ir
Ligand type: Amino-sulfonamide; Cp*
Host protein: Streptavidin (Sav)
Anchoring strategy: Supramolecular
Optimization: Chemical & genetic
Max TON: 3874
ee: 75
PDB: ---
Notes: ---

Neocarzinostatin-Based Hybrid Biocatalysts for Oxidation Reactions

Mahy, J.-P.; Ricoux, R.

Dalton Trans., 2014, 10.1039/c4dt00151f


Metal: Fe
Ligand type: Porphyrin
Anchoring strategy: Supramolecular
Optimization: ---
Reaction: Sulfoxidation
Max TON: 6
ee: 13
PDB: ---
Notes: ---

Oxidation Catalysis via Visible-Light Water Activation of a [Ru(bpy)3]2+ Chromophore BSA–Metallocorrole Couple

Gross, Z.; Mahy, J.-P.

Dalton Trans., 2016, 10.1039/c5dt04158a


Metal: Mn
Ligand type: Corrole
Anchoring strategy: Supramolecular
Optimization: ---
Reaction: Sulfoxidation
Max TON: 21
ee: 16
PDB: ---
Notes: Water as oxygen source

Semi-Synthesis of an Artificial Scandium(III) Enzyme with a β-Helical Bio-Nanotube

Ueno, T.

Dalton Trans., 2012, 10.1039/C2DT31030A


Metal: Sc
Ligand type: Bipyridine
Host protein: [(gp5βf)3]2
Anchoring strategy: Cystein-maleimide
Optimization: Genetic
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Supramolecular Interactions Between Functional Metal Complexes and Proteins

Review

Duhme-Klair, A. K.

Dalton Trans., 2009, 10.1039/b915776j


Notes: ---

Synthesis of Hybrid Transition-Metalloproteins via Thiol-Selective Covalent Anchoring of Rh-Phosphine and Ru-Phenanthroline Complexes

Kamer, P. C. J.; Laan, W.

Dalton Trans., 2010, 10.1039/c0dt00239a


Metal: Rh
Ligand type: COD; Phosphine
Anchoring strategy: Covalent
Optimization: ---
Reaction: Hydrogenation
Max TON: ---
ee: ---
PDB: 2PHY
Notes: ---

(η6-Arene) Ruthenium(II) Complexes and Metallo-Papain Hybrid as Lewis Acid Catalysts of Diels–Alder Reaction in Water

Salmain, M.

Dalton Trans., 2010, 10.1039/c001630f

Covalent embedding of a (η6-arene) ruthenium(II) complex into the protein papain gives rise to a metalloenzyme displaying a catalytic efficiency for a Lewis acid-mediated catalysed Diels–Alder reaction enhanced by two orders of magnitude in water.


Metal: Ru
Ligand type: Benzene; Phenanthroline
Host protein: Papain (PAP)
Anchoring strategy: Covalent
Optimization: Chemical
Max TON: 440
ee: ---
PDB: ---
Notes: TOF = 220 h-1