2 publications

2 publications

Importance of Scaffold Flexibility/Rigidity in the Design and Directed Evolution of Artificial Metallo-β-Lactamases

Song, W. J.; Tezcan, F. A.

J. Am. Chem. Soc., 2017, 10.1021/jacs.7b08981


Metal: Zn
Ligand type: Amino acid
Host protein: Zn8:AB54
Anchoring strategy: Dative
Optimization: Genetic
Reaction: Hydrolysis
Max TON: ---
ee: ---
PDB: 5XZI
Notes: Supramolecular protein scaffold constructed from cytochrome cb562 building blocks, Ampicillin hydrolysis: kcat/KM = 130 min-1 * M-1

Metal: Zn
Ligand type: Amino acid
Host protein: Zn8:AB54 (mutant C96T)
Anchoring strategy: Dative
Optimization: Genetic
Reaction: Hydrolysis
Max TON: ---
ee: ---
PDB: 5XZJ
Notes: Supramolecular protein scaffold constructed from cytochrome cb562 building blocks, Ampicillin hydrolysis: kcat/KM = 210 min-1 * M-1

Importance of Scaffold Flexibility/Rigidity in the Design and Directed Evolution of Artificial Metallo-β-Lactamases

Song, W. J.; Tezcan, F. A.

J. Am. Chem. Soc., 2017, 10.1021/jacs.7b08981


Metal: Zn
Ligand type: Amino acid
Host protein: Zn8:AB54
Anchoring strategy: Dative
Optimization: Genetic
Reaction: Hydrolysis
Max TON: ---
ee: ---
PDB: 5XZI
Notes: Supramolecular protein scaffold constructed from cytochrome cb562 building blocks, Ampicillin hydrolysis: kcat/KM = 130 min-1 * M-1

Metal: Zn
Ligand type: Amino acid
Host protein: Zn8:AB54 (mutant C96T)
Anchoring strategy: Dative
Optimization: Genetic
Reaction: Hydrolysis
Max TON: ---
ee: ---
PDB: 5XZJ
Notes: Supramolecular protein scaffold constructed from cytochrome cb562 building blocks, Ampicillin hydrolysis: kcat/KM = 210 min-1 * M-1