3 publications

3 publications

Hydrolytic Catalysis and Structural Stabilization in a Designed Metalloprotein

Pecoraro, V. L.

Nat. Chem., 2011, 10.1038/NCHEM.1201


Metal: Hg; Zn
Ligand type: Amino acid
Host protein: TRI peptide
Anchoring strategy: Dative
Optimization: Chemical & genetic
Max TON: >10
ee: ---
PDB: 3PBJ
Notes: Zn ion for catalytic activity, Hg ion for structural stability of the ArM. PDB ID 3PBJ = Structure of an analogue.

Metal: Hg; Zn
Ligand type: Amino acid
Host protein: TRI peptide
Anchoring strategy: Dative
Optimization: Chemical & genetic
Max TON: ---
ee: ---
PDB: 3PBJ
Notes: Zn ion for catalytic activity, Hg ion for structural stability of the ArM, kcat/KM ≈ 1.8*105 M-1*s-1. PDB ID 3PBJ = Structure of an analogue.

Hydrolytic Catalysis and Structural Stabilization in a Designed Metalloprotein

Pecoraro, V. L.

Nat. Chem., 2011, 10.1038/NCHEM.1201


Metal: Hg; Zn
Ligand type: Amino acid
Host protein: TRI peptide
Anchoring strategy: Dative
Optimization: Chemical & genetic
Max TON: >10
ee: ---
PDB: 3PBJ
Notes: Zn ion for catalytic activity, Hg ion for structural stability of the ArM. PDB ID 3PBJ = Structure of an analogue.

Metal: Hg; Zn
Ligand type: Amino acid
Host protein: TRI peptide
Anchoring strategy: Dative
Optimization: Chemical & genetic
Max TON: ---
ee: ---
PDB: 3PBJ
Notes: Zn ion for catalytic activity, Hg ion for structural stability of the ArM, kcat/KM ≈ 1.8*105 M-1*s-1. PDB ID 3PBJ = Structure of an analogue.

Influence of Active Site Location on Catalytic Activity in De Novo-Designed Zinc Metalloenzymes

Pecoraro, V. L.

J. Am. Chem. Soc., 2013, 10.1021/ja401537t


Metal: Hg; Zn
Ligand type: Amino acid
Host protein: TRI peptide
Anchoring strategy: Dative
Optimization: Chemical & genetic
Max TON: ---
ee: ---
PDB: 3PBJ
Notes: Influence of position of Zn and Hg ion on catalytic activity of the ArM tested. PDB ID 3PBJ = Structure of an analogue.