3 publications
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A Hydrogenase Model System Based on the Sequence of Cytochrome c: Photochemical Hydrogen Evolution in Aqueous Media
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Chem. Commun. 2011, 47, 8229, 10.1039/c1cc11157d
The diiron carbonyl cluster is held by a native CXXC motif, which includes Cys14 and Cys17, in the cytochrome c sequence. It is found that the diiron carbonyl complex works well as a catalyst for H2 evolution. It has a TON of ∼80 over 2 h at pH 4.7 in the presence of a Ru-photosensitizer and ascorbate as a sacrificial reagent in aqueous media.
Metal: FeLigand type: CarbonylHost protein: Cytochrome cAnchoring strategy: DativeOptimization: ---Notes: Horse heart cytochrome C
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Photoinduced Hydrogen Evolution Catalyzed by a Synthetic Diiron Dithiolate Complex Embedded within a Protein Matrix
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ACS Catal. 2014, 4, 2645-2648, 10.1021/cs500392e
The hydrogen-evolving diiron complex, (μ-S)2Fe2(CO)6 with a tethered maleimide moiety was synthesized and covalently embedded within the cavity of a rigid β-barrel protein matrix by coupling a maleimide moiety to a cysteine residue within the β-barrel. The (μ-S)2Fe2(CO)6 core within the cavity was characterized by UV–vis absorption and a characteristic CO vibration determined by IR measurements. The diiron complex embedded within the cavity retains the necessary catalytic activity (TON up to 130 for 6 h) to evolve H2 via a photocatalytic cycle with a Ru photosensitizer in a solution of 100 mM ascorbate and 50 mM Tris/HCl at pH 4.0 and 25 °C.
Notes: ---
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Regioselective Hydroformylation of Styrene Using Rhodium-Substituted Carbonic Anhydrase
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ChemCatChem 2010, 2, 953-957, 10.1002/cctc.201000159
CA confidential: Replacing the active‐site zinc in carbonic anhydrase (CA) by rhodium forms a new enzymatic catalyst for cofactor‐free hydroformylation of styrene with syn gas. Unlike free rhodium, this rhodium–protein hybrid, [Rh]–CA, is regioselective (8.4:1) for linear over branched aldehyde product, which is a 40‐fold change in regioselectivity compared to free rhodium.
Metal: RhHost protein: Human carbonic anhydrase II (hCAII)Anchoring strategy: Metal substitutionOptimization: GeneticNotes: PDB ID 4CAC = Structure of Zn containing hCAII