6 publications

6 publications

An Artificial Di-Iron Oxo-Orotein with Phenol Oxidase Activity

DeGrado, W. F.; Lombardi, A.

Nat. Chem. Biol., 2009, 10.1038/nchembio.257

Here we report the de novo design and NMR structure of a four-helical bundle di-iron protein with phenol oxidase activity. The introduction of the cofactor-binding and phenol-binding sites required the incorporation of residues that were detrimental to the free energy of folding of the protein. Sufficient stability was, however, obtained by optimizing the sequence of a loop distant from the active site.


Metal: Fe
Ligand type: Amino acid
Host protein: Due Ferro 1
Anchoring strategy: Dative
Optimization: Genetic
Reaction: Alcohol oxidation
Max TON: >50
ee: ---
PDB: 2KIK
Notes: kcat/KM ≈ 1380 M-1*min-1

Metal: Fe
Ligand type: Amino acid
Host protein: Due Ferro 1
Anchoring strategy: Dative
Optimization: Genetic
Reaction: Amine oxidation
Max TON: ---
ee: ---
PDB: 2KIK
Notes: kcat/KM ≈ 83 M-1*min-1

Artificial Diiron Enzymes with a De Novo Designed Four-Helix Bundle Structure

Review

DeGrado, W. F.; Lombardi, A.

Eur. J. Inorg. Chem., 2015, 10.1002/ejic.201500470


Notes: ---

Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials

Lombardi, A.; Nastri, F.

Int. J. Mol. Sci., 2018, 10.3390/ijms19102896


Metal: Fe
Ligand type: Amino acid; Porphyrin
Anchoring strategy: Covalent
Optimization: Chemical & genetic
Reaction: Oxidation
Max TON: ---
ee: ---
PDB: ---
Notes: Immobilization of the ArM on gold surfaces via a lipoic acid anchor.

Design and Engineering of Artificial Oxygen-Activating Metalloenzymes

Review

Lombardi, A.; Lu, Y.

Chem. Soc. Rev., 2016, 10.1039/C5CS00923E


Notes: ---

Diiron-Containing Metalloproteins: Developing Functional Models

Review

DeGrado, W. F.; Lombardi, A.

C. R. Chim., 2007, 10.1016/j.crci.2007.03.010


Notes: ---

Oxidation Catalysis by Iron and Manganese Porphyrins within Enzyme-Like Cages

Review

Lombardi, A.; Maglio, O.; Nastri, F.

Biopolymers, 2018, 10.1002/bip.23107


Notes: ---