Artificial Metalloenzymes Publications @Ward Group

2 publications

An Artificial Metalloenzyme for Carbene Transfer Based on a Biotinylated Dirhodium Anchored Within Streptavidin

Cat. Sci. Technol. 2018, 8, 2294-2298, 10.1039/C8CY00646F

We report an artificial carbenoid transferase which combines a biotinylated dirhodium moiety within streptavidin scaffold.

Metal: Rh

Ligand type: Carboxylate

Host protein: Streptavidin (Sav)

Anchoring strategy: Supramolecular

Optimization: Chemical & genetic

Reaction: Cyclopropanation

Max TON: ~60

ee: ---

PDB: ---

Notes: Cyclopropanation reaction was also performed in the E. coli periplasm.

Metal: Rh

Ligand type: Carboxylate

Host protein: Streptavidin (Sav)

Anchoring strategy: Supramolecular

Optimization: Chemical & genetic

Reaction: C-H insertion

Max TON: ~60

ee: ---

PDB: ---

Notes: ---

Covalent Versus Non-covalent (Biocatalytic) Approaches for Enantioselective Sulfoxidation Catalyzed by Corrole Metal Complexes

Gross, Z.

Cat. Sci. Technol. 2011, 1, 578, 10.1039/c1cy00046b

Oxidation of thioanisoles, catalyzed by chiral manganese(III) and iron(III) corroles, provides the corresponding sulfoxides in moderate chemical yields and low enantioselectivities. Biocatalysis by non-chiral albumin-associated manganese(III) corroles proceeds much better and allows for the enantioselective synthesis of the pharmacologically important R-modafinil, in 88% yield and 73% ee.

Metal: Mn

Ligand type: Corrole

Host protein: Rabbit serum albumin (RSA)

Anchoring strategy: Supramolecular

Optimization: Chemical & genetic

Reaction: Sulfoxidation

Max TON: 45

ee: 70

PDB: ---

Notes: ---