A Structural View of Synthetic Cofactor Integration into [FeFe]-Hydrogenases
Chem. Sci. 2016, 7, 959-968, 10.1039/C5SC03397G
Crystal structures of semisynthetic [FeFe]-hydrogenases with variations in the [2Fe] cluster show little structural differences despite strong effects on activity.
Metal: FeLigand type: CN; CO; DithiolateHost protein: [FeFe]-hydrogenase from C. pasteurianum (CpI)Anchoring strategy: DativeOptimization: ChemicalReaction: H2 evolutionMax TON: ---ee: ---PDB: 4XDCNotes: H2 evolution activity of the ArM: 2874 (mmol H2)*min-1*(mg protein)-1.