Metal-Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes
How to train a protein: Metal‐conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82 % e.r. in the hydrogenation of ketones. The modular setup enables a rapid generation of artificial metalloenzyme libraries, which can be adapted to a broad range of catalytic conditions.
Metal: RhHost protein: Papain (PAP)Anchoring strategy: CovalentOptimization: ChemicalReaction: HydrogenationMax TON: 89ee: 64PDB: ---Notes: ---