Artificial Metalloenzymes Publications @Ward Group

3 publications

Bovine Serum Albumin-Cobalt(II) Schiff Base Complex Hybrid: An Efficient Artificial Metalloenzyme for Enantioselective Sulfoxidation using Hydrogen Peroxide

Bian, H.-D.; Liang, H.

Dalton Trans. 2016, 45, 8061-8072, 10.1039/C5DT04507J

An artificial metalloenzyme (BSA–CoL) based on the incorporation of a cobalt(ii) Schiff base complex {CoL, H2L = 2,2′-[(1,2-ethanediyl)bis(nitrilopropylidyne)]bisphenol} with bovine serum albumin (BSA) has been synthesized and characterized.

Metal: Co

Ligand type: Amine; Phenolate

Host protein: Bovine serum albumin (BSA)

Anchoring strategy: Supramolecular

Optimization: Chemical

Reaction: Sulfoxidation

Max TON: 98

ee: 87

PDB: ---

Notes: ---

Immobilization of an Artificial Imine Reductase Within Silica Nanoparticles Improves its Performance

Shahgaldian, P.; Ward, T.R.

Chem. Commun. 2016, 52, 9462-9465, 10.1039/c6cc04604e

Silica nanoparticles equipped with an artificial imine reductase display remarkable activity towards cyclic imine- and NAD+ reduction. The method, based on immobilization and protection of streptavidin on silica nanoparticles, shields the biotinylated metal cofactor against deactivation yielding over 46 000 turnovers in pure samples and 4000 turnovers in crude cellular extracts.

Metal: Ir

Ligand type: Amino-sulfonamide; Cp*

Host protein: Streptavidin (Sav)

Anchoring strategy: Supramolecular

Optimization: Genetic

Reaction: Transfer hydrogenation

Max TON: 4554

ee: 89

PDB: ---

Notes: Reaction in nanoparticles

Semisynthesis of Bipyridyl-Alanine Cytochrome c Mutants: Novel Proteins with Enhanced Electron-Transfer Properties

Gray, H.B.; Imperiali, B.

J. Am. Chem. Soc. 1993, 115, 8455-8456, 10.1021/ja00071a068

n/a

Metal: Fe; Ru

Ligand type: Bipyridine; Porphyrin

Host protein: Horse heart cytochrome c

Anchoring strategy: Covalent

Optimization: ---

Reaction: Electron transfer

Max TON: ---

ee: ---

PDB: ---

Notes: No catalysis