Transforming Carbonic Anhydrase into Epoxide Synthase by Metal Exchange
Enantioselective epoxidation of styrene was observed in the presence of manganese‐containing carbonic anhydrase as catalyst. The probable oxygen‐transfer reagent is peroxymonocarbonate, which has a structural similarity with the hydrogenocarbonate substrate of the natural reaction. Styrene was chosen as the enzyme possesses a small hydrophobic cavity close to the active site.
Metal: MnLigand type: Amino acidHost protein: Bovine carbonic anhydrase II (CA)Anchoring strategy: Metal substitutionOptimization: Chemical & geneticReaction: EpoxidationMax TON: 4.1ee: 52PDB: ---Notes: ---