Artificial Metalloenzymes Publications @Ward Group

1 publication

Immobilization of Two Organometallic Complexes into a Single Cage to Construct Protein-Based Microcompartment

Chem. Commun. 2016, 52, 5463-5466, 10.1039/C6CC00679E

Natural protein-based microcompartments containing multiple enzymes promote cascade reactions within cells. We use the apo-ferritin protein cage to mimic such biocompartments by immobilizing two organometallic Ir and Pd complexes into the single protein cage. Precise locations of the metals and their accumulation mechanism were studied by X-ray crystallography.

Metal: Ir

Ligand type: Amino acid; Cp*

Host protein: Apo-ferritin

Anchoring strategy: Dative

Optimization: Chemical

Reaction: Hydrogenation

Max TON: ~2

ee: 15

PDB: 5E2D

Notes: Tandem reaction (Hydrogenation and Suzuki-Miyaura coupling) to form biphenylethanol from 4-iodoacetophenone and phenylboronic acid. TON and ee are given for the tandem reaction product.

Metal: Pd

Ligand type: Allyl; Amino acid

Host protein: Apo-ferritin

Anchoring strategy: Dative

Optimization: Chemical

Reaction: Suzuki-Miyaura coupling

Max TON: ~1

ee: 15

PDB: 5E2D

Notes: Tandem reaction (Hydrogenation and Suzuki-Miyaura coupling) to form biphenylethanol from 4-iodoacetophenone and phenylboronic acid.