17 publications

17 publications

An Artificial Metalloenzyme: Creation of a Designed Copper Binding Site in a Thermostable Protein

Reetz, M. T.

Angew. Chem., Int. Ed., 2010, 10.1002/anie.201002106

Guided by nature: A designed binding site comprising the His/His/Asp motif for CuII complexation has been constructed in a robust protein by site‐specific mutagenesis (see picture). The artificial metalloenzyme catalyzes an enantioselective Diels–Alder reaction.


Metal: Cu
Ligand type: Amino acid
Host protein: tHisF
Anchoring strategy: Dative
Optimization: Genetic
Max TON: 6.7
ee: 46
PDB: ---
Notes: ---

Artificial Metalloenzymes

Review

Roelfes, G.

ChemCatChem, 2010, 10.1002/cctc.201000011


Notes: ---

Artificial Metalloenzymes: Enantioselective Catalysis and Beyond

Review

Ward, T. R.

Chimia, 2010, 10.2533/chimia.2010.846


Notes: ---

Artificial Metalloenzymes Through Cysteine-Selective Conjugation of Phosphines to Photoactive Yellow Protein

Kamer, P. C. J.

ChemBioChem, 2010, 10.1002/cbic.201000159


Metal: Pd
Ligand type: Allyl; Phosphine
Anchoring strategy: Covalent
Optimization: Chemical & genetic
Reaction: Allylic amination
Max TON: 45
ee: ---
PDB: 2PHY
Notes: ---

Construction of Robust Bio-Nanotubes using the Controlled Self-Assembly of Component Proteins of Bacteriophage T4

Ueno, T.

Small, 2010, 10.1002/smll.201000772


Metal: Cu
Ligand type: Flavin
Host protein: [(gp5βf)3]2
Anchoring strategy: Lysine-succinimide
Optimization: ---
Reaction: Cycloaddition
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Coordination Chemistry Studies and Peroxidase Activity of a New Artificial Metalloenzyme Built by the “Trojan Horse” Strategy

Mahy, J.-P.

J. Mol. Catal. A: Chem., 2010, 10.1016/j.molcata.2009.10.016


Metal: Fe
Ligand type: Porphyrin
Host protein: Antibody 7A3
Anchoring strategy: Supramolecular
Optimization: ---
Max TON: ---
ee: ---
PDB: ---
Notes: k1 = 574 M-1 * min-1

De Novo Enzymes: From Computational Design to mRNA Display

Review

Seelig, B.

Trends Biotechnol., 2010, 10.1016/j.tibtech.2010.04.003


Notes: ---

Design Strategies for the Creation of Artificial Metalloenzymes

Review

Ward, T. R.

Curr. Opin. Chem. Biol., 2010, 10.1016/j.cbpa.2009.11.026


Notes: ---

Improving the Enantioselectivity of Artificial Transfer Hydrogenases Based on the Biotin–Streptavidin Technology by Combinations of Point Mutations

Ward, T. R.

Inorg. Chim. Acta, 2010, 10.1016/j.ica.2009.02.001


Metal: Ru
Ligand type: Amino-sulfonamide; P-cymene
Host protein: Streptavidin (Sav)
Anchoring strategy: Supramolecular
Optimization: Chemical & genetic
Max TON: 98
ee: 98
PDB: ---
Notes: ---

Metal: Ru
Ligand type: Amino-sulfonamide; Benzene
Host protein: Streptavidin (Sav)
Anchoring strategy: Supramolecular
Optimization: Chemical & genetic
Max TON: 24
ee: 84
PDB: 2QCB
Notes: ---

Introducing a 2-His-1-Glu Nonheme Iron Center into Myoglobin Confers Nitric Oxide Reductase Activity

Lu, Y.

J. Am. Chem. Soc., 2010, 10.1021/ja103516n


Metal: Fe
Ligand type: Amino acid
Host protein: Myoglobin (Mb)
Anchoring strategy: Dative
Optimization: Genetic
Max TON: 320
ee: ---
PDB: 3MN0
Notes: Sperm whale myoglobin

Oxidation of Organic Molecules in Homogeneous Aqueous Solution Catalyzed by Hybrid Biocatalysts (Based on the Trojan Horse Strategy)

Mahy, J.-P.

Tetrahedron: Asymmetry, 2010, 10.1016/j.tetasy.2010.03.050


Metal: Fe
Ligand type: Porphyrin
Host protein: Antibody 7A3
Anchoring strategy: Supramolecular
Optimization: ---
Reaction: Sulfoxidation
Max TON: 9
ee: 10
PDB: ---
Notes: ---

Metal: Mn
Ligand type: Porphyrin
Host protein: Antibody 7A3
Anchoring strategy: Supramolecular
Optimization: ---
Reaction: Epoxidation
Max TON: 105
ee: ---
PDB: ---
Notes: Imidazole as co-catalyst

Protein-Based Hybrid Catalysts-Design and Evolution

Review

Ward, T. R.

Curr. Opin. Biotechnol., 2010, 10.1016/j.copbio.2010.09.004


Notes: ---

Proteins as Host for Enantioselective Catalysis: Artificial Metalloenzymes Based on the Biotin-Streptavidin Technology

Review

Ward, T. R.

Molecular Encapsulation: Organic Reactions in Constrained Systems, 2010, 10.1002/9780470664872.ch13


Notes: Book chapter

Regioselective Hydroformylation of Styrene Using Rhodium-Substituted Carbonic Anhydrase

Kazlauskas, R. J.

ChemCatChem, 2010, 10.1002/cctc.201000159


Metal: Rh
Ligand type: Acac; Carbonyl
Anchoring strategy: Metal substitution
Optimization: Genetic
Reaction: Hydroformylation
Max TON: 298
ee: ---
PDB: 4CAC
Notes: PDB ID 4CAC = Structure of Zn containing hCAII

Roles of Glutamates and Metal Ions in a Rationally Designed Nitric Oxide Reductase Based on Myoglobin

Lu, Y.

Proc. Natl. Acad. Sci. U. S. A., 2010, 10.1073/pnas.1000526107


Metal: Fe
Ligand type: Amino acid
Host protein: Myoglobin (Mb)
Anchoring strategy: Dative
Optimization: Genetic
Reaction: NO reduction
Max TON: ---
ee: ---
PDB: 3M39
Notes: X-ray structure of mutant I107E.

Metal: Cu
Ligand type: Amino acid
Host protein: Myoglobin (Mb)
Anchoring strategy: Dative
Optimization: Genetic
Reaction: NO reduction
Max TON: ---
ee: ---
PDB: 3M3A
Notes: X-ray structure of mutant I107E.

Synthesis of Hybrid Transition-Metalloproteins via Thiol-Selective Covalent Anchoring of Rh-Phosphine and Ru-Phenanthroline Complexes

Kamer, P. C. J.; Laan, W.

Dalton Trans., 2010, 10.1039/c0dt00239a


Metal: Rh
Ligand type: COD; Phosphine
Anchoring strategy: Covalent
Optimization: ---
Reaction: Hydrogenation
Max TON: ---
ee: ---
PDB: 2PHY
Notes: ---

(η6-Arene) Ruthenium(II) Complexes and Metallo-Papain Hybrid as Lewis Acid Catalysts of Diels–Alder Reaction in Water

Salmain, M.

Dalton Trans., 2010, 10.1039/c001630f

Covalent embedding of a (η6-arene) ruthenium(II) complex into the protein papain gives rise to a metalloenzyme displaying a catalytic efficiency for a Lewis acid-mediated catalysed Diels–Alder reaction enhanced by two orders of magnitude in water.


Metal: Ru
Ligand type: Benzene; Phenanthroline
Host protein: Papain (PAP)
Anchoring strategy: Covalent
Optimization: Chemical
Max TON: 440
ee: ---
PDB: ---
Notes: TOF = 220 h-1