A Metal Ion Regulated Artificial Metalloenzyme
Regulation of enzyme activity is essential in living cells. The rapidly increasing number of designer enzymes with new-to-nature activities makes it necessary to develop novel strategies for controlling their catalytic activity. Here we present the development of a metal ion regulated artificial metalloenzyme created by combining two anchoring strategies, covalent and supramolecular, for introducing a regulatory and a catalytic site, respectively. This artificial metalloenzyme is activated in the presence of Fe2+ ions, but only marginally in the presence of Zn2+.
Metal: FeLigand type: BypyridineHost protein: LmrRAnchoring strategy: CovalentOptimization: GeneticReaction: Friedel-Crafts alkylationMax TON: 14ee: 75PDB: ---Notes: ---