7 publications

7 publications

A Rhodium Complex-Linked β-Barrel Protein as a Hybrid Biocatalyst for Phenylacetylene Polymerization

Hayashi, T

Chem. Commun., 2012, 10.1039/C2CC35165J

Our group recently prepared a hybrid catalyst containing a rhodium complex, Rh(Cp)(cod), with a maleimide moiety at the peripheral position of the Cp ligand. This compound was then inserted into a β-barrel protein scaffold of a mutant of aponitrobindin (Q96C) via a covalent linkage. The hybrid protein is found to act as a polymerization catalyst and preferentially yields trans-poly(phenylacetylene) (PPA), although the rhodium complex without the protein scaffold normally produces cis PPA.


Metal: Rh
Ligand type: COD; Cp*
Host protein: Nitrobindin (Nb)
Anchoring strategy: Cystein-maleimide
Optimization: ---
Max TON: ---
ee: ---
PDB: ---
Notes: ---

Asymmetric Hydrogenation with Antibody-Achiral Rhodium Complex

Harada, A.

Org. Biomol. Chem., 2006, 10.1039/B609242J


Metal: Rh
Ligand type: COD; Phosphine
Host protein: Antibody 1G8
Anchoring strategy: Antibody
Optimization: ---
Reaction: Hydrogenation
Max TON: ---
ee: ---
PDB: ---
Notes: ---

A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium–Nitrobindin Metalloprotein

Schwaneberg, U.

ACS Catal., 2018, 10.1021/acscatal.7b04369


Metal: Rh
Ligand type: COD; Cp
Host protein: Nitrobindin variant NB4
Anchoring strategy: Cystein-maleimide
Optimization: ---
Max TON: 3046
ee: ---
PDB: ---
Notes: Calculated in vivo TON assuming 12800 metalloenzymes per E. coli cell

Directed Evolution of Hybrid Enzymes: Evolving Enantioselectivity of an Achiral Rh-Complex Anchored to a Protein

Reetz, M. T.

Chem. Commun., 2006, 10.1039/b610461d


Metal: Rh
Ligand type: COD; Phosphine
Host protein: Streptavidin (Sav)
Anchoring strategy: Supramolecular
Optimization: Genetic
Reaction: Hydrogenation
Max TON: 4500
ee: 65
PDB: ---
Notes: ---

Rhodium-Complex-Linked Hybrid Biocatalyst: Stereo-Controlled Phenylacetylene Polymerization within an Engineered Protein Cavity

ChemCatChem, 2014, 10.1002/cctc.201301055


Metal: Rh
Ligand type: COD; Cp*
Host protein: Nitrobindin (Nb)
Anchoring strategy: Cystein-maleimide
Optimization: Genetic
Max TON: ---
ee: ---
PDB: 3WJC
Notes: ---

Stereoselective Hydrogenation of Olefins Using Rhodium-Substituted Carbonic Anhydrase—A New Reductase

Kazlauskas, R. J.

Chem. - Eur. J., 2008, 10.1002/chem.200801673


Metal: Rh
Ligand type: COD
Anchoring strategy: Metal substitution
Optimization: Genetic
Reaction: Hydrogenation
Max TON: 15.8
ee: ---
PDB: ---
Notes: ---

Metal: Rh
Ligand type: COD
Anchoring strategy: Metal substitution
Optimization: Genetic
Reaction: Hydrogenation
Max TON: 80.5
ee: ---
PDB: 4CAC
Notes: PDB ID 4CAC = Structure of Zn containing hCAII

Synthesis of Hybrid Transition-Metalloproteins via Thiol-Selective Covalent Anchoring of Rh-Phosphine and Ru-Phenanthroline Complexes

Kamer, P. C. J.; Laan, W.

Dalton Trans., 2010, 10.1039/c0dt00239a


Metal: Rh
Ligand type: COD; Phosphine
Anchoring strategy: Covalent
Optimization: ---
Reaction: Hydrogenation
Max TON: ---
ee: ---
PDB: 2PHY
Notes: ---