1 publication

1 publication

Generation of a Functional, Semisynthetic [FeFe]-Hydrogenase in a Photosynthetic Microorganism

Berggren, G.; Lindblad, P.

Energy Environ. Sci. 2018, 11, 3163-3167, 10.1039/C8EE01975D

[FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

Metal: Fe
Ligand type: CN; CO
Anchoring strategy: Reconstitution
Optimization: Chemical & genetic
Reaction: H2 evolution
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