Artificial Dicopper Oxidase: Rational Reprogramming of Bacterial Metallo- b-lactamase into a Catechol Oxidase
Chem. - Asian J. 2012, 7, 1203-1207, 10.1002/asia.201101014
Teaching metalloenzymes new tricks: An artificial type III dicopper oxidase has been developed using a hydrolytic enzyme, metallo‐β‐lactamase, as a metal‐binding platform. The triple mutant D88G/S185H/P224G redesigned by computer‐assisted structural analysis showed spectroscopic features similar to those of type III copper proteins and exhibited a high catalytic activity in the oxidation of catechols under aerobic conditions.
Metal: CuLigand type: Amino acidHost protein: β-lactamaseAnchoring strategy: DativeOptimization: GeneticReaction: Catechol oxidationMax TON: ---ee: ---PDB: 2FU7Notes: ---